Autism Spectrum Disorders-A Review on The Preventive Aspects

Autism Spectrum disorder (ASD) is a neurodevelopmental disorder presenting with defects in social commu-nication skills and repetitive sensory motor behaviour. There has been increasing concern regarding this dis-order due to its increase in incidence and prevalence in recent years. Since it stays for lifelong and has no spe-cific pharmacological treatment, many studies are being conducted to find ways of preventing this disorder. Better understanding of the causative factors will lead to better development of prevention strategies. This review aims at highlighting the methods of prevention of ASD which are mostly focussed on the environmen-tal factors causing ASD as well as the ongoing researches on development of drugs and genetic modification using animal models. Some studies have shown preventive effects of factors such as nutrition supplements such as Vitamin D and folate, breastfeeding and avoiding exposure to harmful chemicals and drugs. The aim is to prevent the occurrence of the disorder and preventing the severity of symptoms wherever possible. Still more studies are needed to establish the exact association between the possible causative factors and ASD so that clinical trials can be done to establish the preventive effects in future.

Results of ala carte Posteromedial Soft Tissue Release in Idiopathic Clubfoot

@#Introduction: The aim of this study is to assess the outcomes of ala carte posteromedial release in children over two years of age who were not responding to the Ponseti method of treatment of idiopathic clubfoot. Materials and Methods: A retrospective observational study from September 2013 to August 2015 was conducted at a tertiary level medical teaching institution. The clubfeet were classified according to the Harold and Walker classification. Radiographic parameters assessed were the talocalcaneal angle (AP, lateral), talus-first metatarsal angle (AP, lateral) and calcaneal-fifth metatarsal angle. The scar and the functional score, according to Laaveg and Ponseti, were evaluated as outcome measures at the final follow-up. Results: Twenty-four children with a mean age of 43.7 ± 24.7 months were enrolled in the study. There was a total of 36 clubfeet: 21 (65.6%) with a poor functional outcome; 12 (37.4%) with excellent to good scar in both horizontal and vertical components. There was a statistical significance between the pre-operative and post-operative radiological parameters (p<0.05). None of the patients presented with any limitation of activities of daily living despite the poor functional outcome in many of the children. There was no significant association between the qualities of scar (horizontal, vertical) and the functional outcome with age at presentation, pre-operative Harold and Walker classification and pre-operative radiographic angles. Conclusion: Surgical intervention in terms of ala carte posteromedial soft tissue release could not produce a good outcome over four years in CTEV. The threshold for surgery in CTEV should be high, given the poor results.

Errors, omissions and medication patterns of handwritten outpatient prescriptions in Bangladesh: a cross-sectional health survey.

Handwritten prescription errors are worldwide common problem. We investigated the types and extent of prescription errors in three cities of Bangladesh. Total 900 outpatient prescriptions were surveyed following the guidelines of WHO and BNF. Prescription errors in superscription, inscription and subscription were analyzed by Microsoft Excel 2010. About 73% patients visited MBBS and 8% BDS doctors. The male outpatients (55%) were slightly higher than female (45%). Commonly found errors of prescription were in superscription part, i.e. gender (64.33%), age (17.67%) and name (5%) of the patients. In inscription part, most common error was the direction for drug use (42%) followed by the dosage regimens (28%), doses (26%), and dosage forms (12.67%) of the drug. In subscription part, illegible handwriting was most common (46%) followed by the prescribers sign (10.67%) and date (4%). In a single prescription, averagely 3 and maximally 8 drugs were found to be prescribed. Most frequently prescribed drugs were antibiotics (18.25%) followed by antacids (17.45%) and NSAIDs (13.60%). Errors in handwritten prescriptions are the primary causes of treatment failure in Bangladesh. More professional care by the prescribers’ and the inclusion of pharmacists in hospital can only reduce the errors and assure safer health.

In vitro explant culture of mantle epithelium of freshwater pearl mussel.

The in vitro culture of nacre secreting pallial mantle explants of freshwater pearl producing mussel, Lamellidens marginalis (Lamarck) included depuration of pearl mussels with different physical and chemical agents to eradicate various commensals, removal of pallial mantle ribbon, aseptic preparation of explants from the ribbon and transfer of those explants into tissue culture petri dishes. Special synthetic tissue culture media enriched with additives viz., inactivated calf fetal serum and antibiotics were poured into plates with explants. The culture plates were incubated at 30 degrees C in a CO2 incubator at 5%, CO2. The cultures could be maintained for 42-45 days without any contamination. After 12 hr epithelial like cells began to migrate out and formed a complete cell sheet surrounding the explant within 12-15 days. The epithelial cells in the culture indicated functional viability as subsequently after 38-40 days of culture, typical aragonitic 'nacre' crystals of CaCO3 could be observed throughout the culture plates.

Inherited factor X deficiency in two brothers.

Two brothers born to same parents were diagnosed with inherited factor X deficiency of severe type. Clinical presentation in both the cases were haemarthrosis. The elder brother was diagnosed in the year 1991 when he was four and half years old. Recently the youngest child in the family also presented with haemarthrosis at age of one and half years. Diagnosis was made by abnormal results of Coagulation factors screening mainly Prothrombin time, Activated partial thromboplastin time, Russell's viper venom test, mixing tests factor X assay. Both the brothers had Factor X activity less than one percent.

Role of S gene product of bacteriophage lambda in host cell lysis.

Studies with the induced lysogens of λ S+R+, λS–R+, λS+R– and λS–R– phages have shown that while the S gene product is essential for the action of intracellular R gene product to release the periplasmic alkaline phosphatase in the presence of EDTA, the latter gene product can bring about this effect while acting on Escherichia coli cells from outside, in the absence of functional S gene product; chloroform, could help the intracellular R gene product in effecting bacterial lysis in the absence of S gene product. These result support the premise that the S gene product facilitates the R gene product in crossing the cytoplasmic membrane into the periplasmic space such that the latter can act on the peptidoglycan layer of the host cell thus causing both the release of alkaline phosphatase and cell lysis.